MetalBindProtPlantV1, Main, Exploration, bibRecord, 000069

Bioaccumulation of Arsenic by Engineered Escherichia coli Cells Expressing Rice Metallothionein Isoforms.

Identifieur interne : 000069 ( Main/Exploration ); précédent : 000068; suivant : 000070

Bioaccumulation of Arsenic by Engineered Escherichia coli Cells Expressing Rice Metallothionein Isoforms.

Auteurs : Azar Shahpiri [Iran] ; Asghar Mohammadzadeh [Iran]

Source :

Current microbiology [ 1432-0991 ] ; 2018.

RBID : pubmed:30151557

Descripteurs français

KwdFr :
- Arsenic (métabolisme), Escherichia coli (génétique), Escherichia coli (métabolisme), Génie génétique (MeSH), Isoformes de protéines (génétique), Isoformes de protéines (métabolisme), Métallothionéine (génétique), Métallothionéine (métabolisme), Oryza (génétique), Protéines recombinantes (génétique), Protéines recombinantes (métabolisme), Protéines végétales (génétique), Protéines végétales (métabolisme).
MESH :
- génétique : Escherichia coli, Isoformes de protéines, Métallothionéine, Oryza, Protéines recombinantes, Protéines végétales.
- métabolisme : Arsenic, Escherichia coli, Isoformes de protéines, Métallothionéine, Protéines recombinantes, Protéines végétales.
- Génie génétique.

English descriptors

KwdEn :
- Arsenic (metabolism), Escherichia coli (genetics), Escherichia coli (metabolism), Genetic Engineering (MeSH), Metallothionein (genetics), Metallothionein (metabolism), Oryza (genetics), Plant Proteins (genetics), Plant Proteins (metabolism), Protein Isoforms (genetics), Protein Isoforms (metabolism), Recombinant Proteins (genetics), Recombinant Proteins (metabolism).
MESH :
- chemical , genetics : Metallothionein, Plant Proteins, Protein Isoforms, Recombinant Proteins.
- chemical , metabolism : Arsenic, Metallothionein, Plant Proteins, Protein Isoforms, Recombinant Proteins.
- genetics : Escherichia coli, Oryza.
- metabolism : Escherichia coli.
- Genetic Engineering.

Abstract

Metallothioneins (MTs) are low-molecular weight cysteine (Cys)-rich proteins with high metal-binding capacity. Based on the Cys arrangement in their amino acid sequences, plant MTs are categorized into four classes. This study evaluated the ability of genetically engineered Escherichia coli cells, which express four rice MT isoforms as fusion proteins with glutathione-S-transferase (GST), to remove arsenic. As compared with control strain, the expression of GST-OsMT1, GST-OsMT2, GST-OsMT3, and GST-OsMT4 resulted in 8-, 5.6-, 3-, and 1.1-fold-higher As³⁺ accumulation. The recombinant GST-OsMT isoforms were purified using affinity chromatography and their apo-forms were prepared. The ability of the GST-OsMT2 isoform to bind with As³⁺ in vitro was also confirmed by ultraviolet (UV) absorption spectra recorded after the reconstitution of apo-proteins with As³⁺. However, the formation of complexes of other MT isoforms with arsenic was not observed.

DOI: 10.1007/s00284-018-1556-3
PubMed: 30151557

Affiliations:

Iran

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Le document en format XML

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<author><name sortKey="Shahpiri, Azar" sort="Shahpiri, Azar" uniqKey="Shahpiri A" first="Azar" last="Shahpiri">Azar Shahpiri</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Biotechnology, College of Agriculture, Isfahan University of Technology, 84156-83111, Isfahan, Iran. a.shahpiri@cc.iut.ac.ir.</nlm:affiliation>
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<author><name sortKey="Mohammadzadeh, Asghar" sort="Mohammadzadeh, Asghar" uniqKey="Mohammadzadeh A" first="Asghar" last="Mohammadzadeh">Asghar Mohammadzadeh</name>
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<author><name sortKey="Mohammadzadeh, Asghar" sort="Mohammadzadeh, Asghar" uniqKey="Mohammadzadeh A" first="Asghar" last="Mohammadzadeh">Asghar Mohammadzadeh</name>
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<term>Escherichia coli (genetics)</term>
<term>Escherichia coli (metabolism)</term>
<term>Genetic Engineering (MeSH)</term>
<term>Metallothionein (genetics)</term>
<term>Metallothionein (metabolism)</term>
<term>Oryza (genetics)</term>
<term>Plant Proteins (genetics)</term>
<term>Plant Proteins (metabolism)</term>
<term>Protein Isoforms (genetics)</term>
<term>Protein Isoforms (metabolism)</term>
<term>Recombinant Proteins (genetics)</term>
<term>Recombinant Proteins (metabolism)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Arsenic (métabolisme)</term>
<term>Escherichia coli (génétique)</term>
<term>Escherichia coli (métabolisme)</term>
<term>Génie génétique (MeSH)</term>
<term>Isoformes de protéines (génétique)</term>
<term>Isoformes de protéines (métabolisme)</term>
<term>Métallothionéine (génétique)</term>
<term>Métallothionéine (métabolisme)</term>
<term>Oryza (génétique)</term>
<term>Protéines recombinantes (génétique)</term>
<term>Protéines recombinantes (métabolisme)</term>
<term>Protéines végétales (génétique)</term>
<term>Protéines végétales (métabolisme)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Metallothionein</term>
<term>Plant Proteins</term>
<term>Protein Isoforms</term>
<term>Recombinant Proteins</term>
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<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Arsenic</term>
<term>Metallothionein</term>
<term>Plant Proteins</term>
<term>Protein Isoforms</term>
<term>Recombinant Proteins</term>
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<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Escherichia coli</term>
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<term>Protéines végétales</term>
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<term>Escherichia coli</term>
<term>Isoformes de protéines</term>
<term>Métallothionéine</term>
<term>Protéines recombinantes</term>
<term>Protéines végétales</term>
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<keywords scheme="MESH" xml:lang="en"><term>Genetic Engineering</term>
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<keywords scheme="MESH" xml:lang="fr"><term>Génie génétique</term>
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<front><div type="abstract" xml:lang="en">Metallothioneins (MTs) are low-molecular weight cysteine (Cys)-rich proteins with high metal-binding capacity. Based on the Cys arrangement in their amino acid sequences, plant MTs are categorized into four classes. This study evaluated the ability of genetically engineered Escherichia coli cells, which express four rice MT isoforms as fusion proteins with glutathione-S-transferase (GST), to remove arsenic. As compared with control strain, the expression of GST-OsMT1, GST-OsMT2, GST-OsMT3, and GST-OsMT4 resulted in 8-, 5.6-, 3-, and 1.1-fold-higher As<sup>3+</sup>
 accumulation. The recombinant GST-OsMT isoforms were purified using affinity chromatography and their apo-forms were prepared. The ability of the GST-OsMT2 isoform to bind with As<sup>3+</sup>
 in vitro was also confirmed by ultraviolet (UV) absorption spectra recorded after the reconstitution of apo-proteins with As<sup>3+</sup>
. However, the formation of complexes of other MT isoforms with arsenic was not observed.</div>
</front>
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<Abstract><AbstractText>Metallothioneins (MTs) are low-molecular weight cysteine (Cys)-rich proteins with high metal-binding capacity. Based on the Cys arrangement in their amino acid sequences, plant MTs are categorized into four classes. This study evaluated the ability of genetically engineered Escherichia coli cells, which express four rice MT isoforms as fusion proteins with glutathione-S-transferase (GST), to remove arsenic. As compared with control strain, the expression of GST-OsMT1, GST-OsMT2, GST-OsMT3, and GST-OsMT4 resulted in 8-, 5.6-, 3-, and 1.1-fold-higher As<sup>3+</sup>
 accumulation. The recombinant GST-OsMT isoforms were purified using affinity chromatography and their apo-forms were prepared. The ability of the GST-OsMT2 isoform to bind with As<sup>3+</sup>
 in vitro was also confirmed by ultraviolet (UV) absorption spectra recorded after the reconstitution of apo-proteins with As<sup>3+</sup>
. However, the formation of complexes of other MT isoforms with arsenic was not observed.</AbstractText>
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<ForeName>Azar</ForeName>
<Initials>A</Initials>
<AffiliationInfo><Affiliation>Department of Biotechnology, College of Agriculture, Isfahan University of Technology, 84156-83111, Isfahan, Iran. a.shahpiri@cc.iut.ac.ir.</Affiliation>
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<Language>eng</Language>
<GrantList CompleteYN="Y"><Grant><GrantID>97000792</GrantID>
<Agency>Iran National Science Foundation</Agency>
<Country></Country>
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Bioaccumulation of Arsenic by Engineered Escherichia coli Cells Expressing Rice Metallothionein Isoforms.

Bioaccumulation of Arsenic by Engineered Escherichia coli Cells Expressing Rice Metallothionein Isoforms.

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English descriptors

Abstract

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